QuanTI-FRET documentation#
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The QuanTI-FRET method proposes calibrating the instrument and the FRET pair to simply calculate absolute FRET probabilities from a triplet of images acquired under the same conditions and with the same FRET pair. All the photophysical and instrumental factors are included in this calibration, leaving the variability of the results to biological origins.
The quanti-fret package provides all the tools to perform first the
calibration, and then to make quantitative FRET measurement of your experiments,
using only your triplet images. It comes either as a Python’s standalone apps or
a Napari Plugin .
The code is publicly available here.
Note
If you use the QuanTI-FRET plug-in in your scientific work, please cite:
Leblanc, J., Lombard, A.H., Saumureau, A. et al. Live-cell quantitative FRET imaging made simple by autocalibration in QuanTI-FRET. Eur. Phys. J. E 48, 74 (2025). https://doi.org/10.1140/epje/s10189-025-00541-z
Note
A test dataset is available here.
Global Workflow
This describes the generic concepts, workflow and naming of QuanTI-FRET. If you are not familiar with the method, this is the place to start with.
GUI Tutorial
This tutorial will guide you step by step on how to use the QuanTI-FRET GUI application through Napari or the standalone app.
CLI Tutorial
This tutorial will guide you step by step on how to use the QuanTI-FRET command line interface (CLI) app.
Utility Tools
QuanTI-FRET comes with some utility tools to either help you in your usage of the main app, or to add other functionalities. These tools are described here.
Developers’ Guide
This is a description of the structure of the QuanTI-FRET code, the interactions between the different components, and the workflow used. It is targeted to people that want to help in the development of the application.
API reference
The API reference containing a detailed description of all the functions, modules, and classes included in QuanTI-FRET.